Gene Cloning and Construction of Expression Vector

Polygalacturonase one of the hydrolytic enzymes involved in the degradation of pectin and cellulose content of the growing tissues in many horticultural plants. CaPG gene is responsible for the production of polygalacturonase in capsicum cultivars, under laid project is associated with the cloning of CaPG gene and construction of many appropriate vectors carries the aim gene in bacteria and other interested commodities. Expression vector termed as pVBG2307 is unique and novel vector offering more broader cloning site with greater than twenty restriction endonucleases.